Laboratorio di Genetica Microbica
Micro4Legumes
Objectives
Develop Synthetic Communities (SynComms) composed by symbiotic nitrogen-fixing rhizobia and nonsymbiotic endophytes with plant-growth promotion activities (auxin production, nitrogen-fixation) for improving growth and resistance of alfalfa to drought conditions
Partners
IBBR-CNR, Naples (Italy)
University of Palermo, Italy)
Activities
WP1 SynComms constitution
1.1 Strain selection
1.2 Testing physiological features of single strains and consorzia
1.3 Genome sequencing and annotation of strains
1.4 Pilot trials of inoculation in vitro
WP2 Efficacy of SynComms
2.1 Set-up of the greenhouse experiment
2.2 Growth evaluation
2.3 Agronomic analyses and yield evaluation
2.4 Identification of the optimal combination of SynComm-plant variety and effect on soil microbiome
WP3 Coordination and Dissemination
3.1 Coordination
3.2 Dissemination
Dissemination
Interviews for local radio broadcast Controradio 20.11.2020: Bright - Notte della ricerca
Round table chat during Bright 2020: Youtube channel UniFI
Symposium at "Accademia dei Georgofili" (Evento Georgofili Leguminose e Agricoltura Sostenibile )
Publications
Fagorzi C., et al (2020) Symbiotic and non-symbiotic members of the genus Ensifer (syn. Sinorhizobium) are separated into two clades based on comparative genomics and high-throughput phenotyping. Genome Biology and Evolution evaa221
diCenzo G.C., et al. (2020) Genome-scale metabolic reconstruction of the symbiosis between a leguminous plant and a nitrogen-fixing bacterium. Nature Communications 11, Article number: 2574
Fagorzi C., et al. (2021) Nonadditive Transcriptomic Signatures of Genotype-by-Genotype Interactions during the Initiation of Plant-Rhizobium Symbiosis. mSystems, 6: e00974-20